Cell shipping

Without cryopreservation
Procedures and protocols

SHIPPING CELL MONOLAYER CULTURES IN PETAKA G3

STEP A (BEFORE SHIPPING)

1. Be sure that the cells were properly prepared for shipment (ATTACHED)
2. Be sure that the cells were 75% confluent immediately before shipping
3. Be sure that the pH of the media was neutral or slightly acidic (orange the media Phenol read indicator)

4. Transfer the Petakas from the incubator to the shipping apackage with the adecuate protection against mechanical damage.

STEP B
5. Avoid, if possible, traveling times longer than 15 days
6. Be sure that the shipment didn't suffer mechanical damaging accidents

STEP C
7. At arrival check under the microscope the cell conditions
8. Live the Petaka resting in horizontal position for at least 2 hours or even
overnight
9. Check under the microscope the cell conditions
10. Withdraw the shipping media completely
11. Check under the microscope the cell conditions
12. Inject carefully 24 mL of new media with additives
13. Incubate at 37OC in HORIZONTAL POSITION until the cells become confluent
14. If both sides are cultured flip the petaka every 12 hours

STEP D
15. Detach the cells with 3 mL of the detaching solution and incubate in horizontal
position following the specific protocol
16.Check cell detachment under the microscope
17.Inject in Petaka G3 7 mL of new media with the appropriate additives according
with the new cell culture protocol
18. Softly shake the Petaka in order to disperse evenly the cells in the media
19.With a Pipette and a 200 microL tip get a sample of the cell suspension and
estimate the cell concentration and cell viability

STEP E
20.Withdraw all media with cells and transfer them dosing the suspension to new
cell culture devices, Petaka G3 or any other device
21.Add new media up to the normal level according with the cell culture device and
protocol.le-click here to edit the text.

Picture.- CHO cells after 10 of traveling at environment conditions in aeroplain and shipping trucks,including customs management.

Celartia
974 Checkrein Av.
Columbus, Ohio 43229
United States

Email: support@celartia.com
Phone: +1 614 844 4900
Mobile: +1 614 975 1922

Preparing adherent cells for shipment

Recovering cells after shipment

Splitting Cells in Petaka without Centrifuge

REMEMBER! Media filling and withdrawing, always holding Petaka in vertical position !

Celartia. Innovation and Progress