SHIPPING SUSPENDED CELL CULTURES IN
PETAKA G3

STEP A (BEFORE SHIPPING)

1. Use preferable Petaka G3 FLAT
2. Be sure that the cells are properly prepared for shipment (25 mL of total
media)
3. Be sure that the cells are viable immediately before shipping
4. Be sure that the pH of the media is neutral or slightly acidic (orange media Phenol read indicator)

5. Transfer the Petakas from the incubator to the shipping package with the adecuate protection against mechanical damage.

STEP B

.
6. Avoid if possible traveling times longer than 15 days
7. Be sure that the shipment didn't suffer mechanical damaging accidents

STEP C

8. At arrival check under the microscope the cell conditions (pick through the
port 200 microL sample and check cell concentration and viability)
9. Softly shake the Petaka in order to disperse evenly the cells in the media
10. With a Pipette-pipettor withdraw and transfer X ml of media with cells to
new Petakas G3 (preferable FLAT) or other device
11.Add to the ne Petakas (or other devices) new media up to 25 mL (or the
normal level according with the cell culture device and protocol).

Picture 1.- Mouse myloma cells (Hybridoma) afsuspended in hybridoma cell culture media. After 10 days of traveling at environment conditions in aeroplain and transport trucks,including customs management. (Viability 75%)

Shipping cells; Safe and easy, without DMSO nor dry ice

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Celartia
974 Checkrein Av.
Columbus, Ohio 43229
United States

Email: support@celartia.com
Phone: +1 614 844 4900
Mobile: +1 614 975 1922

REMEMBER! Media filling and withdrawing, always holding Petaka in vertical position !

Shipping cells in Petaka G3

Method #1

Picture 2.- Human lymphocytes in RPMI 1640 after 7 days of traveling at environment conditions in regular shipping vehicles. (Viability 80%)

RECEIVING CELLS SHIPPED IN PETAKA G3

CELLS SHIPPED IN PETAKA G3

RECEIVING CELLS SHIPPED IN PETAKA G3

Culturing non adherent cells in Petaka G3 bioreactor